Using transcription of six Puccinia triticina races to identify the effective secretome during infection of wheat.
Identifieur interne : 000095 ( Main/Exploration ); précédent : 000094; suivant : 000096Using transcription of six Puccinia triticina races to identify the effective secretome during infection of wheat.
Auteurs : Myron Bruce [États-Unis] ; Kerri A. Neugebauer [États-Unis] ; David L. Joly [Canada] ; Pierre Migeon [États-Unis] ; Christina A. Cuomo [États-Unis] ; Shichen Wang [États-Unis] ; Eduard Akhunov [États-Unis] ; Guus Bakkeren [Canada] ; James A. Kolmer [États-Unis] ; John P. Fellers [États-Unis]Source :
- Frontiers in plant science [ 1664-462X ] ; 2014.
Abstract
Wheat leaf rust, caused by the basidiomycete Puccinia triticina, can cause yield losses of up to 20% in wheat producing regions. During infection, the fungus forms haustoria that secrete proteins into the plant cell and effect changes in plant transcription, metabolism, and defense. It is hypothesized that new races emerge as a result of overcoming plant resistance via changes in the secreted effector proteins. To understand gene expression during infection and find genetic differences associated with races, RNA from wheat leaves infected with six different rust races, at 6 days post inoculation, was sequenced using Illumina. As P. triticina is an obligate biotroph, RNA from both the host and fungi were present and separated by alignment to the P. triticina genome and a wheat EST reference. A total of 222,571 rust contigs were assembled from 165 million reads. An examination of the resulting contigs revealed 532 predicted secreted proteins among the transcripts. Of these, 456 were found in all races. Fifteen genes were found with amino acid changes, corresponding to putative avirulence effectors potentially recognized by 11 different leaf rust resistance (Lr) genes. Twelve of the potential avirulence effectors have no homology to known genes. One gene had significant similarity to cerato-platanin, a known fungal elicitor, and another showed similarity to fungal tyrosinase, an enzyme involved in melanin synthesis. Temporal expression profiles were developed for these genes by qRT-PCR and show that the genes expression patterns were consistent between races from infection initiation to just prior to spore eruption.
DOI: 10.3389/fpls.2013.00520
PubMed: 24454317
PubMed Central: PMC3888938
Affiliations:
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Neugebauer</name><affiliation wicri:level="2"><nlm:affiliation>Department of Plant Pathology, Kansas State University Manhattan, KS, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Plant Pathology, Kansas State University Manhattan, KS</wicri:regionArea><placeName><region type="state">Kansas</region></placeName></affiliation></author><author><name sortKey="Joly, David L" sort="Joly, David L" uniqKey="Joly D" first="David L" last="Joly">David L. Joly</name><affiliation wicri:level="1"><nlm:affiliation>Département de biologie, Université de Moncton Moncton, NB, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Département de biologie, Université de Moncton Moncton, NB</wicri:regionArea><wicri:noRegion>NB</wicri:noRegion></affiliation></author><author><name sortKey="Migeon, Pierre" sort="Migeon, Pierre" uniqKey="Migeon P" first="Pierre" last="Migeon">Pierre Migeon</name><affiliation wicri:level="2"><nlm:affiliation>Department of Plant Pathology, Kansas State University Manhattan, KS, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Plant Pathology, Kansas State University Manhattan, KS</wicri:regionArea><placeName><region type="state">Kansas</region></placeName></affiliation></author><author><name sortKey="Cuomo, Christina A" sort="Cuomo, Christina A" uniqKey="Cuomo C" first="Christina A" last="Cuomo">Christina A. Cuomo</name><affiliation wicri:level="2"><nlm:affiliation>Broad Institute of MIT and Harvard Cambridge, MA, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Broad Institute of MIT and Harvard Cambridge, MA</wicri:regionArea><placeName><region type="state">Massachusetts</region></placeName></affiliation></author><author><name sortKey="Wang, Shichen" sort="Wang, Shichen" uniqKey="Wang S" first="Shichen" last="Wang">Shichen Wang</name><affiliation wicri:level="2"><nlm:affiliation>Department of Plant Pathology, Kansas State University Manhattan, KS, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Plant Pathology, Kansas State University Manhattan, KS</wicri:regionArea><placeName><region type="state">Kansas</region></placeName></affiliation></author><author><name sortKey="Akhunov, Eduard" sort="Akhunov, Eduard" uniqKey="Akhunov E" first="Eduard" last="Akhunov">Eduard Akhunov</name><affiliation wicri:level="2"><nlm:affiliation>Department of Plant Pathology, Kansas State University Manhattan, KS, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Plant Pathology, Kansas State University Manhattan, KS</wicri:regionArea><placeName><region type="state">Kansas</region></placeName></affiliation></author><author><name sortKey="Bakkeren, Guus" sort="Bakkeren, Guus" uniqKey="Bakkeren G" first="Guus" last="Bakkeren">Guus Bakkeren</name><affiliation wicri:level="1"><nlm:affiliation>Pacific Agri-Food Research Centre, Agriculture and Agri-Food Canada Summerland, BC, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Pacific Agri-Food Research Centre, Agriculture and Agri-Food Canada Summerland, BC</wicri:regionArea><wicri:noRegion>BC</wicri:noRegion></affiliation></author><author><name sortKey="Kolmer, James A" sort="Kolmer, James A" uniqKey="Kolmer J" first="James A" last="Kolmer">James A. Kolmer</name><affiliation wicri:level="2"><nlm:affiliation>USDA-ARS Cereal Disease Laboratory, Department of Plant Pathology, University of Minnesota St. Paul, MN, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>USDA-ARS Cereal Disease Laboratory, Department of Plant Pathology, University of Minnesota St. Paul, MN</wicri:regionArea><placeName><region type="state">Minnesota</region></placeName></affiliation></author><author><name sortKey="Fellers, John P" sort="Fellers, John P" uniqKey="Fellers J" first="John P" last="Fellers">John P. Fellers</name><affiliation wicri:level="2"><nlm:affiliation>USDA-ARS Hard Winter Wheat Genetics Research Unit, Department of Plant Pathology Manhattan, KS, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>USDA-ARS Hard Winter Wheat Genetics Research Unit, Department of Plant Pathology Manhattan, KS</wicri:regionArea><placeName><region type="state">Kansas</region></placeName></affiliation></author></analytic><series><title level="j">Frontiers in plant science</title><idno type="ISSN">1664-462X</idno><imprint><date when="2014" type="published">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Wheat leaf rust, caused by the basidiomycete Puccinia triticina, can cause yield losses of up to 20% in wheat producing regions. During infection, the fungus forms haustoria that secrete proteins into the plant cell and effect changes in plant transcription, metabolism, and defense. It is hypothesized that new races emerge as a result of overcoming plant resistance via changes in the secreted effector proteins. To understand gene expression during infection and find genetic differences associated with races, RNA from wheat leaves infected with six different rust races, at 6 days post inoculation, was sequenced using Illumina. As P. triticina is an obligate biotroph, RNA from both the host and fungi were present and separated by alignment to the P. triticina genome and a wheat EST reference. A total of 222,571 rust contigs were assembled from 165 million reads. An examination of the resulting contigs revealed 532 predicted secreted proteins among the transcripts. Of these, 456 were found in all races. Fifteen genes were found with amino acid changes, corresponding to putative avirulence effectors potentially recognized by 11 different leaf rust resistance (Lr) genes. Twelve of the potential avirulence effectors have no homology to known genes. One gene had significant similarity to cerato-platanin, a known fungal elicitor, and another showed similarity to fungal tyrosinase, an enzyme involved in melanin synthesis. Temporal expression profiles were developed for these genes by qRT-PCR and show that the genes expression patterns were consistent between races from infection initiation to just prior to spore eruption. </div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">24454317</PMID><DateCompleted><Year>2014</Year><Month>01</Month><Day>23</Day></DateCompleted><DateRevised><Year>2020</Year><Month>09</Month><Day>30</Day></DateRevised><Article PubModel="Electronic-eCollection"><Journal><ISSN IssnType="Print">1664-462X</ISSN><JournalIssue CitedMedium="Print"><Volume>4</Volume><PubDate><Year>2014</Year><Month>Jan</Month><Day>13</Day></PubDate></JournalIssue><Title>Frontiers in plant science</Title><ISOAbbreviation>Front Plant Sci</ISOAbbreviation></Journal><ArticleTitle>Using transcription of six Puccinia triticina races to identify the effective secretome during infection of wheat.</ArticleTitle><Pagination><MedlinePgn>520</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.3389/fpls.2013.00520</ELocationID><Abstract><AbstractText>Wheat leaf rust, caused by the basidiomycete Puccinia triticina, can cause yield losses of up to 20% in wheat producing regions. During infection, the fungus forms haustoria that secrete proteins into the plant cell and effect changes in plant transcription, metabolism, and defense. It is hypothesized that new races emerge as a result of overcoming plant resistance via changes in the secreted effector proteins. To understand gene expression during infection and find genetic differences associated with races, RNA from wheat leaves infected with six different rust races, at 6 days post inoculation, was sequenced using Illumina. As P. triticina is an obligate biotroph, RNA from both the host and fungi were present and separated by alignment to the P. triticina genome and a wheat EST reference. A total of 222,571 rust contigs were assembled from 165 million reads. An examination of the resulting contigs revealed 532 predicted secreted proteins among the transcripts. Of these, 456 were found in all races. Fifteen genes were found with amino acid changes, corresponding to putative avirulence effectors potentially recognized by 11 different leaf rust resistance (Lr) genes. Twelve of the potential avirulence effectors have no homology to known genes. One gene had significant similarity to cerato-platanin, a known fungal elicitor, and another showed similarity to fungal tyrosinase, an enzyme involved in melanin synthesis. Temporal expression profiles were developed for these genes by qRT-PCR and show that the genes expression patterns were consistent between races from infection initiation to just prior to spore eruption. </AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Bruce</LastName><ForeName>Myron</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>USDA-ARS Hard Winter Wheat Genetics Research Unit, Department of Plant Pathology Manhattan, KS, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Neugebauer</LastName><ForeName>Kerri A</ForeName><Initials>KA</Initials><AffiliationInfo><Affiliation>Department of Plant Pathology, Kansas State University Manhattan, KS, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Joly</LastName><ForeName>David L</ForeName><Initials>DL</Initials><AffiliationInfo><Affiliation>Département de biologie, Université de Moncton Moncton, NB, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Migeon</LastName><ForeName>Pierre</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>Department of Plant Pathology, Kansas State University Manhattan, KS, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Cuomo</LastName><ForeName>Christina A</ForeName><Initials>CA</Initials><AffiliationInfo><Affiliation>Broad Institute of MIT and Harvard Cambridge, MA, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Shichen</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Department of Plant Pathology, Kansas State University Manhattan, KS, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Akhunov</LastName><ForeName>Eduard</ForeName><Initials>E</Initials><AffiliationInfo><Affiliation>Department of Plant Pathology, Kansas State University Manhattan, KS, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bakkeren</LastName><ForeName>Guus</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>Pacific Agri-Food Research Centre, Agriculture and Agri-Food Canada Summerland, BC, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kolmer</LastName><ForeName>James A</ForeName><Initials>JA</Initials><AffiliationInfo><Affiliation>USDA-ARS Cereal Disease Laboratory, Department of Plant Pathology, University of Minnesota St. Paul, MN, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Fellers</LastName><ForeName>John P</ForeName><Initials>JP</Initials><AffiliationInfo><Affiliation>USDA-ARS Hard Winter Wheat Genetics Research Unit, Department of Plant Pathology Manhattan, KS, USA.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2014</Year><Month>01</Month><Day>13</Day></ArticleDate></Article><MedlineJournalInfo><Country>Switzerland</Country><MedlineTA>Front Plant Sci</MedlineTA><NlmUniqueID>101568200</NlmUniqueID><ISSNLinking>1664-462X</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Puccinia triticina</Keyword><Keyword MajorTopicYN="N">RNA sequencing</Keyword><Keyword MajorTopicYN="N">effectors</Keyword><Keyword MajorTopicYN="N">leaf rust</Keyword><Keyword MajorTopicYN="N">secreted peptides</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2013</Year><Month>10</Month><Day>01</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2013</Year><Month>12</Month><Day>02</Day></PubMedPubDate><PubMedPubDate 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Joly</name></noRegion><name sortKey="Bakkeren, Guus" sort="Bakkeren, Guus" uniqKey="Bakkeren G" first="Guus" last="Bakkeren">Guus Bakkeren</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/RustEffectorV1/Data/Main/Exploration
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Ou
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{{Explor lien
|wiki= Bois
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|texte= Using transcription of six Puccinia triticina races to identify the effective secretome during infection of wheat.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:24454317" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
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